Nuclease P1
Product introduction
Nanyang Biotogether nuclease is a 5'-phosphodiesterase derived from Penicillum citrus strain fermentation, after dealing with the biological technology of extraction, microfiltration, ultrafiltration, freeze-drying and refining, finally we get the enzyme preparation. It is the first company in China to prepare nucleases using this method. Nuclease P1 can hydrolyze RNA and DNA exclusively to get 5'-(deoxy) nucleotides. Which is widely used in the industrial production of nucleotides. It is an quite important enzyme preparation.
Product features
n Biological fermentation preparation, safe and reliable
n Advanced purification technology, enzyme activity more than 50,000 u/g, good stability.
n The key technology was rewarded the 2nd prize of 2015 national technology invention.
The largest and advanced bio-manufacturing line from Yeast-Ribonucleic acid-Nucleotides
Properties on yeast lytic activity of Nuclease P1"Nanyang Biotogether”
n The optimum reaction pH is 5.5, stable at 4.6-7.6
n The optimum reaction temperature is 70°C, stable at 65°C-75°C.
n The nuclease which is light yellow powder, no peculiar smell, high hydrolysis efficiency, and has better solubility and fluidity, easy operation.
n Specific hydrolyzed RNA to CMP, UMP, GMP, AMP, and the hydrolysis rate can reach more than 90%.
Nuclease P1 stability technology
The main enzymatic properties such as enzyme activity, stability and content of hybrid enzymes have obvious advantages compared to the world's largest nuclease producing P1 company.
. Biotogether . Amano enzyme
Product applications
n Industrial production of 5'- nucleotides (UMP, CMP, AMP, GMP).
n Industrial production of natural flavour enhancer high nucleotides extract.
n The production of feed nucleotide.
Product usage method:
Yeast: Hydrolysis temperature 70°C, PH 4.0-7.0, nuclease enzyme added 0.2 % - 0.3 %( according to the yeast weight), hydrolysis time 8-10 hours.
RNA: Hydrolysis temperature 70°C, PH 4.0-7.0, Substrate concentration RNA 1%-5%, nuclease enzyme added 3 % - 5 %( according to the substrate RNA), hydrolysis time 4-5 hours.
Product Indexes：
Total colony | Moisture | Heavy metal | Pathogenic bacteria | |
≤50000 cfu/g | ≤10% | ≤10 ppm | Cannot be detected |
Packing: 25kg/barrel, food grade PE bag inside while circular cardboard bucket outside.
1kg package, food grade aluminum foil bag inside, circular cardboard bucket outside.
Storage: Place in a cool, dry place to keep out moisture.
Shelf life: 24 months from the date of production without opening.
